RESUMO
Three serologic test methods were used to study immunologic responses of 36 children vaccinated against smallpox. Following successful primary vaccination, 97 percent of the children converted for neutralizing and HI antibodies and 18 percent for CF antibodies, 3 of 33 children had a 4-fold or greater decrease for neutralizing antibodies, but 29 of 33 children had a decrease for HI antibodies. All children became negative for CF antibodies. After the second vaccination 15 months later in which only one child failed to respond clinically, 78 percent of the children had a 4-fold or greater neutralizing antibody rise; in contrast only 18 per cent showed a significant HI antibody titer increase; 44 per cent converted for CF antibodies. (AU)
Assuntos
Humanos , Pré-Escolar , Criança , Masculino , Feminino , Anticorpos/análise , Vacina Antivariólica/imunologia , Vacinação , Testes de Fixação de Complemento , Testes de Inibição da Hemaglutinação , Esquemas de Imunização , Jamaica , Testes de Neutralização , Vacina AntivariólicaRESUMO
Primary vaccination by intradermal jet injection, using diluted smallpox vaccine, was compared with multiple-pressure vaccination in 625 Jamaican children. The cutaneous and antibody response patterns were evaluated. The primary take rates among those jet vaccinated were 97 percent or more in those receiving vaccines with a titre of 10 6.3 TCID50/ml and 10 7.0 TCID50/ml, and 96 percent in those vaccinated by multiple pressure, using undiluted vaccine. The primary take rates in subjects receiving jet-injected vaccine with titre of 10 6.0 TCID50/ml and 10 5.0 TCID50/ml were 90 percent and 62 percent respectively. Among subjects tested who developed Jennerian vesicles, there was also a failure of neutralizing-antibody development. Vesicle and scar sizes were generally smaller in the jet-vaccinated subjects than in those vaccinated by the multiple-pressure technique. Infants tolerated jet vaccination without difficulty. Vaccinial complications did not occur in any subject. The intradermal jet injection of 0.1ml of vaccine with a titre 10 6.3 TCID 50/ml or higher is recommended as a highly effective method for achieving successful primary smallpox vaccination. The method appears best suited for use in mass smallpox-vaccination programmes.(AU)
